CROT Antikörper

Produktnummer ABIN7214298

Der Kaninchen Polyklonal anti-CROT Antikörper wird verwendet zum Nachweis von CROT in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für CROT Antikörper (ABIN7214298)

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CROT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CROT Antikörper

Verwendungszweck: Rabbit Anti-COT Polyclonal Antibody

Spezifität: COT Polyclonal Antibody detects endogenous levels of COT protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cot around the non-phosphorylation site of T290

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CROT

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Andere Bezeichnung: COT

Hintergrund: MAP3K8, COT, ESTF, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2MAP3K8 is an oncogene that encodes a member of the serine/threonine protein kinase family. Mitogen-activated protein kinase 8 localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.

Molekulargewicht: 60kD

Gen-ID: 1326

UniProt: P41279

Pathways: Monocarboxylic Acid Catabolic Process