PRKAA1/PRKAA2 Antikörper (pThr172, pThr183)

Produktnummer ABIN7201115

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch PRKAA1/PRKAA2 in WB, ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein und Affe.

Kurzübersicht für PRKAA1/PRKAA2 Antikörper (pThr172, pThr183) (ABIN7201115)

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Reaktivität: Human, Maus, Ratte, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAA1/PRKAA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PRKAA1/PRKAA2 Antikörper

Bindungsspezifität: pThr172, pThr183

Verwendungszweck: AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

Spezifität: Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human AMPKalpha1/2 Phospho-Thr183/172

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Andere Bezeichnung: AMPKalpha1/2

Hintergrund: Rabbit Anti-AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody,PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA2, AMPK,Protein kinase AMP-activated catalytic subunit alpha 1 encoded by PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.,5'-AMP-activated protein kinase catalytic subunit alpha-1

Gen-ID: 5562, 5563