Adducin alpha/beta Antikörper

Produktnummer ABIN7213490

Der Kaninchen Polyklonal anti-Adducin alpha/beta Antikörper (ABIN7213490) detektiert spezifisch Adducin alpha/beta in ELISA, IF, IHC, WB und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Adducin alpha/beta Antikörper (ABIN7213490)

Target: Adducin alpha/beta

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails

Verwendungszweck: Rabbit Anti-Adducin α/β Polyclonal Antibody

Spezifität: Adducin α/β Polyclonal Antibody detects endogenous levels of Adducin α/β protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Adducin alpha/beta around the non-phosphorylation site of S726/713

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IP 2-5 μg:mg lysate,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: Adducin alpha/beta

Hintergrund: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha, ADD2, ADDB, Beta-adducin, Erythrocyte adducin subunit betaAdducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca (2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.

Molekulargewicht: 80kD

Gen-ID: 118, 119

UniProt: P35611, P35612