ADD2 Antikörper (AA 440-520)

Produktnummer ABIN7213492

Dieser Anti-ADD2 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von ADD2 in WB, ELISA und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ADD2 Antikörper (AA 440-520) (ABIN7213492)

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-ADD2 Antikörper

Bindungsspezifität: AA 440-520

Verwendungszweck: Adducin β Polyclonal Antibody

Spezifität: Adducin β Polyclonal Antibody detects endogenous levels of Adducin β protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Adducin beta at AA range: 440-520

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu ADD2

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Andere Bezeichnung: Adducin beta

Hintergrund: Rabbit Anti-Adducin β Polyclonal Antibody,ADD2, ADDB, Beta-adducin, Erythrocyte adducin subunit beta,Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.,Beta-adducin

Gen-ID: 119

UniProt: P35612

Pathways: Regulation of Actin Filament Polymerization