ACTN1 Antikörper (AA 1-80)

Produktnummer ABIN7225622

Der Kaninchen Polyklonal anti-ACTN1 Antikörper wird verwendet zum Nachweis von ACTN1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für ACTN1 Antikörper (AA 1-80) (ABIN7225622)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACTN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ACTN1 Antikörper

Bindungsspezifität: AA 1-80

Verwendungszweck: Rabbit Anti-Actinin-α1 Polyclonal Antibody

Spezifität: Actinin-α1 Polyclonal Antibody detects endogenous levels of Actinin-α1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Actinin-alpha1 at AA range: 1-80

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Andere Bezeichnung: Actinin-alpha1

Hintergrund: ACTN1, Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 (actinin alpha 1) encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1.

Molekulargewicht: 103kD

Gen-ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization