CD4 Antikörper

Produktnummer ABIN7200645

Der Maus Monoklonal anti-CD4 Antikörper wird verwendet zum Nachweis von CD4 in Proben von Human, Rhesusaffen und Cynomolgus. Er wurde validiert für FACS.

Kurzübersicht für CD4 Antikörper (ABIN7200645)

Target: CD4

Reaktivität: Human, Rhesusaffen, Cynomolgus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Güteklasse: GMP Grade

Klon: SK3

Produktdetails anti-CD4 Antikörper

Verwendungszweck: CD4 Unconjugated Antibody

Produktmerkmale: The clone SK3, a mouse monoclonal antibody selectively binds with a 55-59kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD4 contains four extracellular Ig-like domains (D1-D4). The epitope for SK3 is located within the D3 domain of the protein, which has a structure resembling an Ig variable domain. Expression of CD4 is observed in subsets of T lymphocytes, monocytes, macrophages, and dendritic cells. Through interaction of MHC-II, CD4 facilitates cell-cell interaction, thymic differentiation, and activation of downstream signaling cascades. HIV infection of T-cells is instigated through binding of HIV to CD4. Binding of SK3 antibody blocks HIV infection through CD4 and also blocks the mixed lymphocyte reaction (MLR).

Aufreinigung: Purified

Reinheit: >95 %

Immunogen: Human peripheral blood T cells

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5µg (unconjugated) of these products are sufficient for staining 1 million cells in a 100µl staining volume or 100µl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS pH 7.2, 0.1 % (w/v) BSA, 0.09 % (w/v) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Gen-ID: 920

NCBI Accession: NM_000616

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events