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CD45 Antikörper (AA 378-577)

Name: CD45 Antikörper (AA 378-577)
Brand: antibodies-online
SKU: ABIN7193646
Price: 640.59 EUR
Availability: InStock

PTPRC Reaktivität: Human FACS, IHC, WB, ELISA, ICC Wirt: Maus Monoclonal 2H3E2 unconjugated

Flow cytometric analysis of Hela cells using CD45R mouse mAb (green) and negative control (red). (CD45 Antikörper (AA 378-577))

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD45R mouse mAb with DAB staining. (CD45 Antikörper (AA 378-577))

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (CD45 Antikörper (AA 378-577))

ELISA

Western blot analysis using CD45R mAb against human CD45R (AA: extra 378-577) recombinant protein. (CD45 Antikörper (AA 378-577))

Western blot analysis using CD45R mAb against HEK293-6e (1) and CD45R (AA: extra 378-577)-hIgGFc transfected HEK293-6e (2) cell lysate. (CD45 Antikörper (AA 378-577))

Western blot analysis using CD45R mouse mAb against Jurkat (1), Rat Serum (2), and mouse Serum (3) cell lysate. (CD45 Antikörper (AA 378-577))

Flow cytometric analysis of Jurkat cells using CD45R mouse mAb (green) and negative control (red). (CD45 Antikörper (AA 378-577))

FACS

Immunofluorescence analysis of Hela cells using CD45R mouse mAb (green). (CD45 Antikörper (AA 378-577))

Immunohistochemical analysis of paraffin-embedded tonsil tissues using CD45R mouse mAb with DAB staining. (CD45 Antikörper (AA 378-577))

IHC

9 Abbildungen

Produktnummer ABIN7193646

Datenblatt (PDF)

Target Alle CD45 (PTPRC) Antikörper anzeigen

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
Bindungsspezifität
16

16

14

11

10

9

6

6

6

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 378-577
Reaktivität
378

246

93

33

20

19

16

14

10

10

7

6

6

4

3

3

2

1

1

1

1

1

1

1

1
Human
Wirt
318

190

126

2

1

1
Maus
Passende Sekundärantikörper
Klonalität
516

115

1
Monoklonal
Konjugat
189

60

56

45

44

16

13

11

11

11

10

10

8

7

7

6

5

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

1

1
Dieser CD45 Antikörper ist unkonjugiert
Applikation
531

188

187

131

126

58

56

50

41

27

18

15

14

13

13

10

7

5

5

4

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Verwendungszweck

CD45R Antibody

Aufreinigung

Purified antibody

Immunogen

Purified recombinant fragment of human CD45R (AA: 378-577) expressed in E. Coli.

Klon

2H3E2

Isotyp

IgG1

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Applikationshinweise

ELISA: 1/10000
FCM: 1/200-1/400
ICC: 1/200 - 1/1000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Andere Bezeichnung

CD45R (PTPRC Produkte)

Hintergrund

Description:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Aliases: PTPRC, LCA, LY5, B220, L-CA, T200, CD45, GP180

Molekulargewicht

147.5kDa

Gen-ID

5788

UniProt

P08575

Pathways

T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling