WT1 Antikörper (AA 1-181)

Produktnummer ABIN7193614

Dieses Maus Monoklonal-Antikörper erkennt spezifisch WT1 in IHC, FACS, WB, ELISA und ICC. Er zeigt eine Reaktivität gegenüber Human und Affe.

Kurzübersicht für WT1 Antikörper (AA 1-181) (ABIN7193614)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Klon: 7F9E8

Produktdetails anti-WT1 Antikörper

Bindungsspezifität: AA 1-181

Verwendungszweck: WT1 Antibody

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200-1/400

ICC: 1/200 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1

Hintergrund:

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Molekulargewicht: 49.2 kDa

UniProt: P19544

Pathways: Tube Formation