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Nanog Antikörper (AA 1-305)

Name: Nanog Antikörper (AA 1-305)
Brand: antibodies-online
SKU: ABIN7155816
Price: 462 EUR
Availability: InStock

Der Maus Monoklonal Anti-Nanog-Antikörper wurde für WB, ELISA, IF, FACS und ICC validiert. Er ist geeignet, Nanog in Proben von Human zu detektieren.

Western Blot Positive WB detected in: Rat brain tissue, Mouse brain tissue All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 42 kDa (Nanog Antikörper (AA 1-305))

Overlay histogram showing Ntera-2 cells stained with ABIN7155816 (red line) at 1:250. (Nanog Antikörper (AA 1-305))

FACS

Western Blot Positive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysate All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 40 kDa (Nanog Antikörper (AA 1-305))

Immunocytochemistry analysis of ABIN7155816 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. (Nanog Antikörper (AA 1-305))

ICC

Immunocytochemistry analysis of ABIN7155816 diluted at 1:100 and staining in Ntera-2 cells performed on a Leica BondTM system. (Nanog Antikörper (AA 1-305))

ICC

Immunofluorescence staining of Hela cells with ABIN7155816 at 1:100, counter-stained with DAPI. (Nanog Antikörper (AA 1-305))

Immunofluorescence staining of Ntera-2 cells with ABIN7155816 at 1:100, counter-stained with DAPI. (Nanog Antikörper (AA 1-305))

Overlay histogram showing Hela cells stained with ABIN7155816 (red line) at 1:250. (Nanog Antikörper (AA 1-305))

FACS

Overlay histogram showing MCF-7 cells stained with ABIN7155816 (red line) at 1:250. (Nanog Antikörper (AA 1-305))

FACS

9 Abbildungen

Produktnummer ABIN7155816

Datenblatt (PDF)

Kurzübersicht für Nanog Antikörper (AA 1-305) (ABIN7155816)

Target

Alle Nanog (NANOG) Antikörper anzeigen

Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität

Human

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser Nanog Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon

8A1D11

Discover our top product NANOG Primärantikörper

Bindungsspezifität
16

16

9

9

9

7

7

6

5

5

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-305

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Protein G purified

Immunogen

Recombinant Human Homeobox protein NANOG protein (1-305AA)

Isotyp

IgG1, IgG1 kappa
anti-Nanog Homeobox (NANOG) antibody
NANOG Reaktivität: Human WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 101-200) antibody
NANOG Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 71-170) antibody
NANOG Reaktivität: Human, Maus WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG Reaktivität: Human WB, ELISA, IF, IHC, FACS Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-100) antibody
NANOG Reaktivität: Human WB Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG Reaktivität: Human WB, ELISA, ICC Wirt: Maus Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG Reaktivität: Human WB, ELISA, IF, IHC Wirt: Ziege Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 267-292) antibody
NANOG Reaktivität: Human WB, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung

NANOG

Hintergrund

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Aliases: Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG

UniProt

Q9H9S0

Pathways

Stem Cell Maintenance