Rekombinanter RNF20 Antikörper

Produktnummer ABIN7127796

Der Kaninchen-Monoklonal Anti-RNF20-Antikörper wurde für WB, ELISA, IF und IHC validiert. Er ist geeignet zum Nachweis von RNF20 in Proben aus Human. }

Kurzübersicht für Rekombinanter RNF20 Antikörper (ABIN7127796)

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RNF20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: 2H12

Produktdetails anti-RNF20 Antikörper

Verwendungszweck: RNF20 Recombinant Monoclonal Antibody

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human RNF20

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Andere Bezeichnung: RNF20

Hintergrund:

Background: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B, reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Aliases: E3 ubiquitin-protein ligase BRE1A (BRE1-A) (hBRE1) (EC 2.3.2.27) (RING finger protein 20) (RING-type E3 ubiquitin transferase BRE1A), RNF20, BRE1A

UniProt: Q5VTR2