KRT5 & KRT6 Antikörper

Produktnummer ABIN7127253

Dieser Maus Monoklonal Antikörper detektiert spezifisch KRT5 & KRT6 in IHC (f). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für KRT5 & KRT6 Antikörper (ABIN7127253)

Target: KRT5 & KRT6

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails

Spezifität: It recognizes polypeptides of 58 kDa and 56 kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma. It does not react with pulmonary adenocarcinoma.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human KRT5 protein

Isotyp: IgG1, IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or skin tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Antigendetails

Target: KRT5 & KRT6

Hintergrund: CK5, Keratin-5, KRT5, Cytoskeletal 6A, KRT6A,Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 58kDa (KRT5), 56kDa (KRT6A)