RBM8A Antikörper

Produktnummer ABIN7127238

Dieses Maus Monoklonal-Antikörper erkennt spezifisch RBM8A in WB, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für RBM8A Antikörper (ABIN7127238)

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RBM8A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-RBM8A Antikörper

Spezifität: Y14 Antibody is a high quality monoclonal Y14 antibody (also designated Y14 antibody) suitable for the detection of the Y14 protein of mouse, rat, human and Xenopus laevis origin. Y14 Antibody (4C4) is available as both the non-conjugated anti-Y14 antibody form, as well as multiple conjugated forms of anti-Y14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human RBM8A protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu RBM8A

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Andere Bezeichnung: RBM8A

Hintergrund: Binder of OVCA1-1, BOV1A, BOV1B, BOV1C, HSPC114, MDS014, ZRNP1, Ribonucleoprotein RBM8A, RNA-binding protein 8A, RNA-binding protein Y14,Y14 / RBM8A
Cellular localisation: Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.

Molekulargewicht: 24kDa

Gen-ID: 9939, 728824

UniProt: Q9Y5S9