CD151 Antikörper

Produktnummer ABIN7127232

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD151 in WB, FACS, IF und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD151 Antikörper (ABIN7127232)

Target: CD151

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD151 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-CD151 Antikörper

Spezifität: CD151 is involved in a wide variety of cell biological processes, including cell adhesion and the transport of integrins via vesicles. The human CD151 gene maps to chromosome 11p15.5 and encodes a 253 amino acid protein, which belongs to the tetraspan (4TM) superfamily. CD151 can associate with several integrin chains including 1, 3, 4, i±2, i±3, i±5 and i±6 integrins. CD151 may provide a framework for the spatial organization of both type I and type II hemidesmosomes, which are specialized junctional complexes that function as cell attachment sites for binding to basement membranes. CD151 RNA transcript (1.6 kb) can be detected in MO7e cells, bone marrow stromal cells, C11 endothelial cells, HUVEC and several myeloid leukemia cell lines, however, no transcript is detected in brain and the lymphoblastoid cell lines MOLT-4 and BALM-1. Leu 149-Glu 213 of CD151 is the interface through which Integrin i±3/ 1 can bind. CD151 can enhance cell motility, invasion and metastasis of cancer cells in a focal adhesion kinase dependent manner.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: The details of the immunogen for this antibody are not available.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil or spleen. HT1080 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD151

Target: CD151

Andere Bezeichnung: CD151

Hintergrund: GP27, hemidesmosomal tetraspanin CD151, Membrane glycoprotein SFA-1, MER2, Platelet endothelial tetraspan antigen 3 (PETA3), platelet surface glycoprotein gp27, Platelet-endothelial cell tetraspan antigen 3, Platelet-endothelial tetraspan antigen 3, RAPH, Red blood cell antigen MER2, SFA1, Tetraspanin-24 (TSPAN24),CD151
Cellular localisation: Cell Surface

Molekulargewicht: 28-32kDa

Gen-ID: 977, 654379

UniProt: P48509