CD63 Antikörper

Produktnummer ABIN7127224

Der Maus-Monoklonal Anti-CD63-Antikörper wurde für FACS, IF, ELISA, IP, IHC (f) und Func validiert. Er ist geeignet zum Nachweis von CD63 in Proben aus Human. }

Kurzübersicht für CD63 Antikörper (ABIN7127224)

Target: CD63

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD63 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Functional Studies (Func)

Produktdetails anti-CD63 Antikörper

Spezifität: CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Kreuzreaktivität (Details): Human. Porcine.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full-length human CD63 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA; Functional studies; Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)); ,Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HL60, THP-1 or NIH/3T3 cells. Human spleen, melanoma or lymphoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD63

Target: CD63

Andere Bezeichnung: CD63

Hintergrund: Gp55, granulophysin, Lysosomal-associated membrane protein 3, Mast cell antigen AD1Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, NGA, Ocular melanoma-associated antigen, Tetraspanin-30, TSPAN30,CD63 (Late Endosomes Marker)
Cellular localisation: Cell surface. Cytoplasm.

Molekulargewicht: 26kDa (core protein), 30-60kDa (glycosylated)

Gen-ID: 967, 445570

UniProt: P08962