QKI Antikörper

Produktnummer ABIN7127192

Der Maus Monoklonal anti-QKI Antikörper (Klon PCRP-QKI-2F10) (ABIN7127192) detektiert spezifisch QKI in WB, IF, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für QKI Antikörper (ABIN7127192)

Target: QKI (Protein Quaking (QKI))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser QKI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Klon: PCRP-QKI-2F10

Produktdetails anti-QKI Antikörper

Verwendungszweck: QKI / Protein Quaking Antibody

Spezifität: QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human QKI protein

Isotyp: IgG2c

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (concentration assay dependent), Flow Cytometry (1-2 μg/million cells),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: U87, HeLa, K562 or MCF7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu QKI

Target: QKI (Protein Quaking (QKI))

Andere Bezeichnung: QKI

Hintergrund:

Homolog of mouse quaking QKI KH domain RNA binding protein, HQK1, Protein quaking, QK1, QK3, QKI, QKI1, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK

HeLa, K562 or MCF7 cells., U87

Molekulargewicht: 37.6kDa

Gen-ID: 9444, 510324

UniProt: Q96PU8