MED21 Antikörper

Produktnummer ABIN7127181

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MED21 in WB, ELISA, IF, Coat, FACS und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MED21 Antikörper (ABIN7127181)

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MED21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunoprecipitation (IP)

Produktdetails anti-MED21 Antikörper

Spezifität: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MED21 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Andere Bezeichnung: MED21

Hintergrund: Forkhead box protein Q1, MED21, Hepatocyte nuclear factor 3 forkhead homolog 1, HFH1, HNF3/forkhead like protein 1, MGC77469, Q1, Winged helix / forkhead transcription factor, zgc:77469,MED21 / SRB7
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm.

Molekulargewicht: 15.5kDa

Gen-ID: 9412, 286145

UniProt: Q13503

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha