ADIPOQ Antikörper

Produktnummer ABIN7127173

Der Maus Monoklonal Anti-ADIPOQ-Antikörper wurde für ELISA, Coat und IHC (f) validiert. Er ist geeignet, ADIPOQ in Proben von Human zu detektieren.

Kurzübersicht für ADIPOQ Antikörper (ABIN7127173)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ADIPOQ Antikörper ist unkonjugiert

Applikation: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-ADIPOQ Antikörper

Spezifität: This MAb reacts with adiponectin, an adipocytokine. Adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma adiponectin levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than in men, which may be due to androgens suppressing adiponectin levels. Furthermore, adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism. Clone ADPN/4256 is used to detect plasma adiponectin concentrations. Clone ADPN/4256 is also used in a combination ELISA with clone ADPN/4255, acting as the capture antibody.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to the tail region of the Adiponectin molecule.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, use antibody without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Andere Bezeichnung: ADIPOQ

Hintergrund: Adiponectin, Adipocyte complement-related 30 kDa protein (ACRP30), Adipocyte-specific secretory protein, Adiponectin, C1Q and collagen domain containing (ACDC), ADIPOQ, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM-1, Gelatin-binding protein 28 (GBP28),Adiponectin (Marker of Obesity)
Cellular localisation: Cytoplasmic & Cell Surface

Molekulargewicht: 26-30kDa

Gen-ID: 9370, 80485

UniProt: Q15848