Rekombinanter p63 Antikörper (AA 1-100)

Produktnummer ABIN7127111

Der Kaninchen Monoklonal anti-p63 Antikörper (Klon P40-4396R) (ABIN7127111) detektiert spezifisch p63 in IHC, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter p63 Antikörper (AA 1-100) (ABIN7127111)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser p63 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: P40-4396R

Produktdetails anti-p63 Antikörper

Bindungsspezifität: AA 1-100

Verwendungszweck: Recombinant p40 (Squamous, Basal & Myoepithelial Cell Marker) Antibody

Spezifität: P40 antibody (antibody recognizing Np63 only) is an isoform of p63, is thought to function as a stem cell factor, responsible for maintaining cells in an uncommitted state with regenerative potential a role that may be recapitulated in tumors derived from these cells. p40 is normally expressed in the basal or progenitor cell layer of stratified epithelia (eg. squamous, urothelial, bronchial), basal cells of some glandular epithelia (eg. prostate), as well as myoepithelial cells of breast and salivary glands, trophoblasts and thymic epithelial cells. In tumor tissues, p40 expression is specific for squamous cell carcinoma. p40 is equivalent to p63 in sensitivity for lung squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. p40 appears to be a more reliable marker for squamous cell carcinoma.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified by Protein A Column.

Immunogen: Recombinant fragment (around aa1-100) of human p40 protein (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293, MDA-MB453 or MCF7 cells. Human prostate, lung or bladder squamous cell carcinoma tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Andere Bezeichnung: TP63

Hintergrund:

Amplified in squamous cell carcinoma (AIS), Chronic ulcerative stomatitis protein (CUSP), EEC3, TP73L, LMS, TP63, Keratinocyte transcription factor KET, NBP, P51/P63, p53 like transcription factor, RHS, SHFM4, TAp63alpha, TP53CP, TP53L, Transformation-related protein 63, Trp53rp1, Trp6,3, Tumor protein p53-like, tumor protein p73-like

HEK293, lung or bladder squamous cell carcinoma tissue., MDA-MB453 or MCF7 cells. Human prostate

Molekulargewicht: 63kDa

Gen-ID: 8626, 137569

UniProt: Q9H3D4