CA9 Antikörper (AA 314-410)

Produktnummer ABIN7127066

Dieses Anti-CA9-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CA9 in IHC, FACS und IHC (f). Geeignet für Human.

Kurzübersicht für CA9 Antikörper (AA 314-410) (ABIN7127066)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CA9 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-CA9 Antikörper

Bindungsspezifität: AA 314-410

Spezifität: Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human CA9 protein fragment (around aa 314-410) (exact sequence is proprietary)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U87 cells. Normal kidney or renal cell carcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Andere Bezeichnung: CA9

Hintergrund: CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250,Renal Cell Carcinoma (Carbonic Anhydrase IX)
Cellular localisation: Cell Surface. Cytoplasmic.

Molekulargewicht: 55kDa

Gen-ID: 768, 63287

UniProt: Q16790