Rekombinanter VWF Antikörper (AA 1150-1250)

Produktnummer ABIN7127055

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch VWF in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter VWF Antikörper (AA 1150-1250) (ABIN7127055)

Target: VWF (Von Willebrand Factor (VWF))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser VWF Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: VWF-4384R

Produktdetails anti-VWF Antikörper

Bindungsspezifität: AA 1150-1250

Verwendungszweck: Recombinant von Willebrand Factor/ Factor VIII Related-Ag (Endothelial Marker) Antibody

Spezifität: Human von Willebrand factor (VWFor factor VIII-related antigen) is a 309 kDa multimeric plasma glycoprotein, important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Von Willebrand factor is synthesized by endothelial cells and is reported to be expressed in a number of tumors of vascular origin. IHC results aid in the classification of acute myeloid leukemia FAB type M7, angiosarcoma and epithelioid hemangioendothelioma.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 1150-1250) of human vWF protein (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HUVEC cells. Human tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu VWF

Target: VWF (Von Willebrand Factor (VWF))

Andere Bezeichnung: VWF

Hintergrund:

Coagulation Factor VIII, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

HUVEC cells. Human tonsil.

Molekulargewicht: 250kDa

Gen-ID: 7450, 440848

UniProt: P04275