Rekombinanter TIA1 Antikörper

Produktnummer ABIN7127002

Produktdetails anti-TIA1 Antikörper

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser TIA1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: The T cell intracellular antigen 1 (TIA 1) is a 17 kDa cytoplasmic granule associated protein also designated as GMP 17, for granule membrane protein of 17 kDa. The GMP 17/TIA 1 Molecule is expressed in cells possessing cytolytic potential and could be involved in the signaling cascade of Fas (CD95) mediated apoptosis. Within hematopoietic cell lines, the TIA1/1352R monoclonal antibody reacts with about 90 % of CD16+, 50 60 % of CD8+, and less than 10 % of CD4+ normal peripheral blood lymphocytes. It reacts with almost all monocytes and granulocytes. This antibody also reacts with CD4+ activated T cell clones, activated NK cell clones, and Con activated thymocytes, but not with B lymphocytes or B cell lines. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human bone marrow malignant cells from a non B, non T acute leukemia

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, spleen and lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Andere Bezeichnung: TIA1 (TIA1 Produkte)

Synonyme: tia1 antikoerper, 2310050N03Rik antikoerper, AI256674 antikoerper, TIA-1 antikoerper, mTIA-1 antikoerper, WDM antikoerper, TIAL1 antikoerper, TIAR antikoerper, tia-1 antikoerper, nucleolysin TIA-1 isoform p40 antikoerper, hypothetical protein antikoerper, nucleolysin TIAR antikoerper, nucleolysin tia-1 antikoerper, nucleolysin TIA-1 antikoerper, cytotoxic granule-associated RNA binding protein 1 antikoerper, TIA1 cytotoxic granule associated RNA binding protein antikoerper, TIA1 cytotoxic granule-associated RNA binding protein antikoerper, TIA1 cytotoxic granule-associated RNA binding protein L homeolog antikoerper, PTRG_10065 antikoerper, PGTG_08590 antikoerper, LOC5568274 antikoerper, CpipJ_CPIJ013665 antikoerper, VDBG_07004 antikoerper, MGYG_03339 antikoerper, Tsp_15456 antikoerper, tia1 antikoerper, Tia1 antikoerper, TIA1 antikoerper, tia1.L antikoerper

Hintergrund: Cytotoxic granule associated RNA binding protein 1, mTIA-1, Nucleolysin TIA1 isoform p40, p40-TIA-1, RNA binding protein TIA1, TIAL1, TIAR, WDM,TIA1 (T-Cell-Restricted Intracellular Antigen-1)
Cellular localisation: Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.

Molekulargewicht: 40kDa

Gen-ID: 7072, 413123

UniProt: P31483