Osteopontin Antikörper

Produktnummer ABIN7126977

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch Osteopontin in WB, FACS, IF, Func und IHC (f). Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für Osteopontin Antikörper (ABIN7126977)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reaktivität: Ratte

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Osteopontin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: OSP-4589

Produktdetails anti-Osteopontin Antikörper

Spezifität: Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

Kreuzreaktivität (Details): Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length rat Spp1 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Functional Studies, Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Bone. Found in plasma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Andere Bezeichnung: SPP1

Hintergrund: BNSP, Bone sialoprotein 1 (BSPI/BSP1), Early T lymphocyte activation 1 (ETA1), Nephropontin, immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (SPP1), CALPHA1 fusion, Urinary stone protein, Uropontin,Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1)
Cellular localisation: Secreted.

Molekulargewicht: 34.9kDa

Gen-ID: 6696, 313

UniProt: P10451

Pathways: Regulation of Cell Size