RORC Antikörper

Produktnummer ABIN7126903

Produktdetails anti-RORC Antikörper

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RORC Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: This MAb recognizes a protein of 63 kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors RORi± and RORi³ are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50 % amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered orphan receptors, due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human RORC protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells) (3 % PFA-fixed cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MOLT4 cells. Human lymphocytes. Human liver or skeletal muscle tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu RORC

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Andere Bezeichnung: RORC (RORC Produkte)

Synonyme: NR1F3 antikoerper, RORG antikoerper, RZR-GAMMA antikoerper, RZRG antikoerper, TOR antikoerper, Nr1f3 antikoerper, RORgamma antikoerper, Thor antikoerper, RAR related orphan receptor C antikoerper, RAR-related orphan receptor C antikoerper, RAR-related orphan receptor gamma antikoerper, RORC antikoerper, Rorc antikoerper

Hintergrund: Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3 (NR1F3), RAR related orphan receptor C, Retinoid-related orphan receptor-gamma (RORG), Rorc, RZR GAMMA, RZRG, TOR,ROR-gamma / RORC (RAR-related Orphan Receptor C)
Cellular localisation: Nuclear

Molekulargewicht: 63kDa

Gen-ID: 6097

UniProt: P51449

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway