Actin Antikörper

Produktnummer ABIN7126863

Der Maus Monoklonal Anti-Actin-Antikörper wurde für WB, IF, FACS und IHC (f) validiert. Er ist geeignet, Actin in Proben von Human zu detektieren.

Kurzübersicht für Actin Antikörper (ABIN7126863)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Actin Antikörper

Spezifität: All eukaryotic cells express Actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Kreuzreaktivität (Details): Human.Predicted to show a broad reactivity.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human ACTA1 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Skeletal muscle.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Andere Bezeichnung: ACTA1

Hintergrund: A actin, ACTA, ACTA1, Actin, alpha skeletal muscle, ACTS_HUMAN, Alpha actin 1, Alpha-actin-1, ASMA, MPFD, Skeletal muscle alpha actin 1,Actin alpha 1, Skeletal Muscle
Cellular localisation: Cytoplasm.

Molekulargewicht: 43kDa

Gen-ID: 58, 1288

UniProt: P68133

Pathways: Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development