MBNL3 Antikörper

Produktnummer ABIN7126833

Produktdetails anti-MBNL3 Antikörper

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MBNL3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Spezifität: Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The muscleblind proteins, MBNL1, MBNL2 and MBNL3, promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors bound to distinct intronic sites. MBNL1 and 2, which associate with expanded CUG repeats in vitro, localize to the nuclear foci in both DM1 and DM2 (myotonic dystrophy types 1 and 2), suggesting that the nuclear accumulation of mutant RNA is pathogenic in DM1, therefore implicating MBNL1 and 2 in the pathogenesis of both disorders. MBNL3, a 354 amino acid protein, inhibits expression of muscle differentiation, opposite to the function of MBNL1, which functions as a promoter of muscle differentiation. MBNL3 shows strong expression in placenta.

Kreuzreaktivität (Details): Human

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MBNL3 protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MBNL3

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Andere Bezeichnung: MBNL3 (MBNL3 Produkte)

Hintergrund: CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, Muscleblind like protein 3, Muscleblind like splicing regulator 3, Muscleblind-like X-linked protein, Protein HCHCR, RP23-98P13.1,MBNL3
Cellular localisation: Nucleus. Cytoplasm. Greater concentration in the nucleus. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts.

Molekulargewicht: 39kDa

Gen-ID: 55796

UniProt: Q9NUK0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development