FERMT1 Antikörper (AA 216-677)

Produktnummer ABIN7126830

Der Maus Monoklonal anti-FERMT1 Antikörper wird verwendet zum Nachweis von FERMT1 in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für FERMT1 Antikörper (AA 216-677) (ABIN7126830)

Target: FERMT1 (Fermitin Family Member 1 (FERMT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FERMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 4A5-14

Produktdetails anti-FERMT1 Antikörper

Bindungsspezifität: AA 216-677

Verwendungszweck: Kindlin-1 / KIND1 Antibody

Spezifität: Kindlin-1 (also known as KIND1, KINDLERIN, UNC112-RELATED PROTEIN 1 (URP1) is an important component of cell-extracellular matrix adhesion. Kindlin-1 is involved in cell adhesion, possibly via its interaction with integrins and may mediate TGF-beta 1 signaling during tumor progression. Kindlin-1 has been shown to be significantly upregulated in lung and colon carcinomas. Mutations in the kindlin-1 gene, FERMT1, causes Kindler syndrome. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin. We have developed monoclonal anti-kidlin-1 antibodies. This monoclonal antibody recognizes kindlin-1 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant protein containing Kindlin-1 (residues 216-677)

Isotyp: IgG2a, lambda

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: 293T whole cell lysate. Human tonsil or liver.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu FERMT1

Target: FERMT1 (Fermitin Family Member 1 (FERMT1))

Andere Bezeichnung: FERMT1

Hintergrund:

Fermitin family homolog 1 (Fermt1), Kindlin-1 (KIND1), Kindlerin, Unc-112, UNC112A, URP1, DTGCU2

293T whole cell lysate. Human tonsil or liver.

Molekulargewicht: 77kDa

Gen-ID: 55612, 472054

UniProt: Q9BQL6