PRMT6 Antikörper

Produktnummer ABIN7126821

Der Maus Monoklonal Anti-PRMT6-Antikörper wurde für IF, IP und FACS validiert. Er ist geeignet, PRMT6 in Proben von Human zu detektieren.

Kurzübersicht für PRMT6 Antikörper (ABIN7126821)

Target: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRMT6 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-PRMT6 Antikörper

Spezifität: A class of proteins termed type 1 protein arginine N-methyltransferase (PRMT) enzymes contribute to posttranslational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties and subcellular localization. PRMTs contain an S-adenosylmethione motif which functions to add one or two methyl groups to guanidino nitrogens of arginine (R) side chains. PRMT6, also known as HRMT1L6, is a nuclear protein belonging to the PRMT family and is predominantly expressed in testis and kidney. It is known to methylate Histones H3, H4 and H2A. PRMT6 is the major dimethyltransferase for Histone H3 and specifically methylates Histone H3 at R2. Methylation at Histone H3 R2 acts to inhibit Histone H3 K4 trimethylation and ultimately leads to the transcriptional repression of genes that are activated by Histone H3 K4 trimethylation. In addition, PRMT6 methylates HIV TAT, possibly functioning as a form of cellular innate immunity to restrict levels of HIV replication.

Kreuzreaktivität (Details): Human. Predicted to react with Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PRMT6 protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF7 or HepG2 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu PRMT6

Target: PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))

Andere Bezeichnung: PRMT6

Hintergrund: Chromobox protein homolog 7, Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6, Histone-arginine N-methyltransferase PRMT6, HMT1 hnRNP methyltransferase like 6., HRMT1L6,PRMT6
Cellular localisation: Nucleus.

Molekulargewicht: 42kDa

Gen-ID: 55170, 26006

UniProt: Q96LA8