OCT4 Antikörper (AA 1-134)

Produktnummer ABIN7126815

Der Maus Monoklonal anti-OCT4 Antikörper wird verwendet zum Nachweis von OCT4 in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für OCT4 Antikörper (AA 1-134) (ABIN7126815)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: Oct4-3508

Produktdetails anti-OCT4 Antikörper

Bindungsspezifität: AA 1-134

Verwendungszweck: OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody

Spezifität: OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells, its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

Kreuzreaktivität (Details): Human

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Raji or U-937 cells. Human seminoma or testicular carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: POU5F1

Hintergrund:

OCT3, OCT3A, OCT4, OCT4A, OCT-3/4, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, octamer-binding transcription factor-3, OTF-3, OTF3, OTF4, PO5F1, POU class 5 homeobox 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, POU5F1

Raji or U-937 cells. Human seminoma or testicular carcinoma. NCCIT.

Molekulargewicht: 38.6kDa

Gen-ID: 5460, 249184

UniProt: Q01860

Pathways: Stem Cell Maintenance