Rekombinanter ATRX Antikörper

Produktnummer ABIN7126814

Dieser Maus Monoklonal Antikörper detektiert spezifisch ATRX in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter ATRX Antikörper (ABIN7126814)

Target: ATRX (helicase 2, X-linked (ATRX))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATRX Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: RATRX-3446

Produktdetails anti-ATRX Antikörper

Verwendungszweck: Recombinant ATRX / RAD54 (Alpha Thalassemia Mental Retardation) Antibody

Spezifität: ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 °™ to mediate chromosomal segregation. Mutations in the ATRX gene correlate with a high incidence of severe X-linked form of syndromal mental retardation associated with °™ thalassemia or ATRX syndrome

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human ATRX protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Raji, HeLa or A431 cells. Pancreas, Adrenal, Ovary, Kidney, Prostate or Stomach.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Andere Bezeichnung: ATRX

Hintergrund:

Alpha thalassemia/mental retardation syndrome X linked homolog, ATP-dependent helicase ATRX, ATR2, ATRX, DNA-dependent ATPase and helicase, MRXHF1, RAD54, RAD54L, SFM1, SHS, Transcriptional regulator ATRX, X-linked helicase II, X-linked nuclear protein, XH2, XNP, Znf-HX

adrenal, HeLa or A431 cells. Human pancreas, Kidney, ovary, prostate or stomach., Raji

Molekulargewicht: 280kDa

Gen-ID: 546, 533526, 653797

UniProt: P46100