Rekombinanter MUC3A Antikörper

Produktnummer ABIN7126724

Dieser Maus Monoklonal Antikörper detektiert spezifisch MUC3A in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter MUC3A Antikörper (ABIN7126724)

Target: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC3A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: RMUC3-1154

Produktdetails anti-MUC3A Antikörper

Verwendungszweck: Recombinant MUC3 (Mucin 3) Antibody

Spezifität: It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues.The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human MUC3A protein (exact sequence is proprietary)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: LS174T cells.Human colon or colon carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MUC3A

Target: MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))

Andere Bezeichnung: MUC3A

Hintergrund:

Intestinal mucin-3A, intestinal mucin-like, MUC3, MUC3A, MUC3B, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin 3B, cell surface associated

LS174T cells.Human colon or colon carcinoma.

Molekulargewicht: 1100kDa

Gen-ID: 4584, 57876

UniProt: Q02505