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Rekombinanter MUC1 Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MUC1 in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN7126721

Kurzübersicht für Rekombinanter MUC1 Antikörper (ABIN7126721)

Target

Alle MUC1 Antikörper anzeigen
MUC1 (Mucin 1 (MUC1))

Antikörpertyp

Recombinant Antibody

Reaktivität

  • 365
  • 77
  • 38
  • 16
  • 6
  • 6
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Human

Wirt

  • 244
  • 146
  • 3
  • 1
  • 1
Maus

Klonalität

  • 216
  • 169
  • 9
Monoklonal

Konjugat

  • 198
  • 32
  • 19
  • 14
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 8
  • 7
  • 7
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Dieser MUC1 Antikörper ist unkonjugiert

Applikation

  • 211
  • 200
  • 139
  • 105
  • 81
  • 78
  • 47
  • 47
  • 44
  • 23
  • 19
  • 16
  • 14
  • 13
  • 13
  • 6
  • 6
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
  • Spezifität

    In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

    Kreuzreaktivität (Details)

    Human.

    Aufreinigung

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogen

    Recombinant full-length human MUC1 protein

    Isotyp

    IgG2a
  • Applikationshinweise

    Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian or endometrial carcinoma.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Konservierungsmittel

    Azide free

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Haltbarkeit

    24 months
  • Target

    MUC1 (Mucin 1 (MUC1))

    Andere Bezeichnung

    MUC1

    Hintergrund

    Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin,MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)
    Cellular localisation: Cytoplasmic & Cell Surface

    Molekulargewicht

    265-400kDa

    Gen-ID

    4582, 89603

    UniProt

    P15941

    Pathways

    Negative Regulation of intrinsic apoptotic Signaling
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