Rekombinanter MUC1 Antikörper

Produktnummer ABIN7126720

Dieses Anti-MUC1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MUC1 in WB und IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter MUC1 Antikörper (ABIN7126720)

Target: MUC1 (Mucin 1 (MUC1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-MUC1 Antikörper

Spezifität: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Kreuzreaktivität (Details): Human and Mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MUC1 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: MUC1

Hintergrund: Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen,MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)
Cellular localisation: Cytoplasmic and cell surface

Molekulargewicht: 265-400kDa

Gen-ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling