MIF Antikörper

Produktnummer ABIN7126691

Produktdetails anti-MIF Antikörper

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MIF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Macrophage migration inhibitory factor, known as MIF or glycosylationinhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor i± (TNFi±), interleukin-1 (IL-1) and interferon-i³ (IFN-i³). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human MIF protein (exact sequence is proprietary)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, thymus or prostate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Andere Bezeichnung: MIF (MIF Produkte)

Hintergrund: GIF, GLIF, Glycosylation inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Macrophage migration inhibitory factor (glycosylation-inhibiting factor), MIF, MIF protein, MMIF, Phenylpyruvate tautomerase,MIF (Macrophage Migration Inhibitory Factor)
Cellular localisation: Secreted. Cytoplasm.

Molekulargewicht: 13kDa

Gen-ID: 4282, 407995

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling