Rekombinanter EpCAM Antikörper (Cytoplasmic Domain)

Produktnummer ABIN7126663

Dieses Anti-EpCAM-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von EpCAM in IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter EpCAM Antikörper (Cytoplasmic Domain) (ABIN7126663)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: AA 289-314, Cytoplasmic Domain

Spezifität: Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Kreuzreaktivität (Details): Human.Dog. Cat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment from cytoplasmic domain (around aa 289-314) of human EpCAM (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HT29 cells. Human colon or breast.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: TACSTD1

Hintergrund: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1),EpCAM / CD326 (Cytoplasmic Domain) (Epithelial Marker)
Cellular localisation: Cell surface

Molekulargewicht: 40-43kDa

Gen-ID: 4072, 542050

UniProt: P16422