KRT14 Antikörper (AA 351-472)

Produktnummer ABIN7126627

Dieses Anti-KRT14-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KRT14 in IHC (f). Geeignet für Human.

Kurzübersicht für KRT14 Antikörper (AA 351-472) (ABIN7126627)

Target: KRT14 (Keratin 14 (KRT14))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT14 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-KRT14 Antikörper

Bindungsspezifität: AA 351-472

Spezifität: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa351-472) of human KRT14 protein (exact sequence is proprietary)

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human skin or squamous cell carcinoma tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu KRT14

Target: KRT14 (Keratin 14 (KRT14))

Andere Bezeichnung: KRT14

Hintergrund: CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, Keratin Type I Cytoskeletal 14, Koebner, NFJ,Cytokeratin 14 (KRT14) (Squamous Cell Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 50kDa

Gen-ID: 3861, 6545380

UniProt: P02533