Cytokeratin 13 Antikörper

Produktnummer ABIN7126625

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 13 in WB, IF und IHC (f). Er zeigt eine Reaktivität gegenüber Kaninchen.

Kurzübersicht für Cytokeratin 13 Antikörper (ABIN7126625)

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reaktivität: Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Cytokeratin 13 Antikörper

Spezifität: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs)that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 13 belongs to the intermediate filament family and is a heterotetramer of two type I acidic and two type II basic keratins. It is generally associated with Cytokeratin 4. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder which predominantly affects noncornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa.

Kreuzreaktivität (Details): Human, mouse, rat and rabbit.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: esophageal keratins of rabbit origin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or A431 cells. Skin or Squamous cell carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Andere Bezeichnung: KRT13

Hintergrund: CK13, Cytokeratin-13, Keratin Type I Cytoskeletal 13, Keratin-13, KRT13, Type I Cytoskeletal 13, WSN2,Cytokeratin 13 (Non-Keratinized Squamous Epithelial Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 52kDa

Gen-ID: 3860, 654550

UniProt: P13646