PDX1 Antikörper

Produktnummer ABIN7126596

Der Maus Monoklonal Anti-PDX1-Antikörper wurde für IF und FACS validiert. Er ist geeignet, PDX1 in Proben von Human zu detektieren.

Kurzübersicht für PDX1 Antikörper (ABIN7126596)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDX1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-PDX1 Antikörper

Spezifität: Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic i²-cells. PDX-1 is a key regulator of pancreatic islet development and Insulin gene transcription in i²-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3i², HNF-1i± and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by Glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of i²-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.

Kreuzreaktivität (Details): Human

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PDX1 protein

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HEK293 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Andere Bezeichnung: PDX1

Hintergrund: Glucose sensitive factor, GSF, IDX1, Insulin promoter factor 1 homeodomain transcription factor, IPF1, STF1, IUF1, Islet/duodenum homeobox 1, MODY4, Pancreas/duodenum homeobox, Somatostatin transactivating factor 1,Pancreatic duodenal homeobox-1 protein (PDX1)
Cellular localisation: Nucleus.

Molekulargewicht: 46kDa

Gen-ID: 3651, 32938

UniProt: P52945

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location