HOXB2 Antikörper (AA 114-220)

Produktnummer ABIN7126513

Dieses Anti-HOXB2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HOXB2 in WB, ELISA, IP, IF, Coat, FACS und IHC (f). Geeignet für Human.

Kurzübersicht für HOXB2 Antikörper (AA 114-220) (ABIN7126513)

Target: HOXB2 (Homeobox B2 (HOXB2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HOXB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-HOXB2 Antikörper

Bindungsspezifität: AA 114-220

Spezifität: Hox genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene HoxB1 is critical to hindbrain development and has phenotypic features frequently observed in autism. Analysis of expression and targeted disruption of HoxB1 demonstrates that it is also essential for patterning progenitor cells along the entire DV axis of rhombomere 4 (r4). HoxB1 maintains this function by acting very early during hindbrain neurogenesis to specify effectors of the Sonic hedgehog and Mash1 signaling pathways. HoxB2 is a homeodomain protein important in neural development that is also expressed during erythropoiesis, hindbrain development and normal human adult lung development. HoxB2 may modulate the amount of gamma-globin mRNA expressed during development and differentiation. In addition, HoxB2 plays an important role in the patterning of hindbrain and pharyngeal arches in the zebrafish.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (aa114-220) of human HOXB2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu HOXB2

Target: HOXB2 (Homeobox B2 (HOXB2))

Andere Bezeichnung: HOXB2

Hintergrund: Homeo box 2H, homeo box B2, homeobox B2, Homeobox protein Hox-2.8, Homeobox protein Hox-2H, Homeobox protein Hox-B2, Hox 2 8, Hox 2H, HOX2, HOX2H, hoxb2, HXB2_HUMAN, K8, K8 home protein,HOXB2 (Transcription Factor)
Cellular localisation: Nucleus. Nucleoplasm.

Molekulargewicht: 37.9kDa

Gen-ID: 3212, 514289

UniProt: P14652