GLUL Antikörper (AA 50-250)

Produktnummer ABIN7126480

Der Maus Monoklonal anti-GLUL Antikörper (Klon GLUL-6600) (ABIN7126480) detektiert spezifisch GLUL in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh) und Pferd.

Kurzübersicht für GLUL Antikörper (AA 50-250) (ABIN7126480)

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GLUL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: GLUL-6600

Produktdetails anti-GLUL Antikörper

Bindungsspezifität: AA 50-250

Verwendungszweck: Glutamine Synthetase / GLUL Antibody

Spezifität: Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Kreuzreaktivität (Details): Human. Horse. Cow.

Aufreinigung: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human brain, stomach or thyroid. A-431 or Y79 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu GLUL

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Andere Bezeichnung: GLUL

Hintergrund:

cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS

Human Liver, Mouse Liver, Rat Liver, Human Brain, Mouse Brain, Rat Brain and Y79

Molekulargewicht: 42kDa

Gen-ID: 2752, 518525

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion