GATA3 Antikörper

Produktnummer ABIN7126462

Dieses Anti-GATA3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GATA3 in WB, FACS, IF und IHC (f). Geeignet für Human.

Kurzübersicht für GATA3 Antikörper (ABIN7126462)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GATA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-GATA3 Antikörper

Spezifität: GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50 kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67 % of urothelial Carcinomas, but none of prostate or renal carcinomas.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human GATA-3 protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human breast or urothelial carcinoma tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Andere Bezeichnung: GATA3

Hintergrund: GATA binding protein-3, GATA-binding factor 3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-3,GATA-3 (Center) (Breast and Urothelial Marker)
Cellular localisation: Nuclear

Molekulargewicht: 50kDa

Gen-ID: 2625, 524134

UniProt: P23771

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation