CNOT10 Antikörper

Produktnummer ABIN7126458

Der Maus Monoklonal anti-CNOT10 Antikörper wird verwendet zum Nachweis von CNOT10 in Proben von Human. Er wurde validiert für WB, ELISA, IF und FACS.

Kurzübersicht für CNOT10 Antikörper (ABIN7126458)

Target: CNOT10 (CCR4-NOT Transcription Complex, Subunit 10 (CNOT10))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CNOT10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: PCRP-CNOT10-1D5

Produktdetails anti-CNOT10 Antikörper

Verwendungszweck: CCR4-NOT transcription complex, subunit 10 (CNOT10) Antibody

Spezifität: CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CNOT10 protein

Isotyp: IgG2c

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CNOT10

Target: CNOT10 (CCR4-NOT Transcription Complex, Subunit 10 (CNOT10))

Andere Bezeichnung: CNOT10

Hintergrund:

FLJ12890, FLJ13165

HeLa or HepG2 cells.

Molekulargewicht: 82.31kDa

Gen-ID: 25904, 444851

UniProt: Q9H9A5