SIRT3 Antikörper

Produktnummer ABIN7126428

Der Maus Monoklonal Anti-SIRT3-Antikörper wurde für WB, ELISA, IP, IF, FACS und Coat validiert. Er ist geeignet, SIRT3 in Proben von Human zu detektieren.

Kurzübersicht für SIRT3 Antikörper (ABIN7126428)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SIRT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Produktdetails anti-SIRT3 Antikörper

Spezifität: Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human SIRT3 protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Andere Bezeichnung: SIRT3

Hintergrund: HSIRT3, Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase, NAD dependent deacetylase sirtuin 3 mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3, SIR2-like protein 3, SIR2L3, Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae), Sirtuin type 3, Sirtuin3,Sirtuin 3 (SIRT3)
Cellular localisation: Mitochondrion matrix.

Molekulargewicht: 43.57kDa

Gen-ID: 23410, 716456

UniProt: Q9NTG7