Rekombinanter MLANA Antikörper (N-Term)

Produktnummer ABIN7126408

Dieses Anti-MLANA-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von MLANA in WB und IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter MLANA Antikörper (N-Term) (ABIN7126408)

Target: MLANA (Melan A (MLANA))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MLANA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-MLANA Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment corresponding to the N-terminus of human Melan A protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: COLO-38, SK-MEL-13 or SK-MEL-19 melanoma cell lines. Human skin or melanoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MLANA

Target: MLANA (Melan A (MLANA))

Andere Bezeichnung: MLANA

Hintergrund: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, MLANA,MART-1 / Melan-A / MLANA (Melanoma Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 20-22kDa (doublet)

Gen-ID: 2315, 154069

UniProt: Q16655