SIRT2 Antikörper

Produktnummer ABIN7126393

Produktdetails anti-SIRT2 Antikörper

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SIRT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Spezifität: The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in a NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT proteins may function via mono-ADP-ribosylation of proteins. SIRT2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human SIRT2 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7 or U-87 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Andere Bezeichnung: SIRT2 (SIRT2 Produkte)

Hintergrund: NAD-dependent deacetylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, Silencing information regulator 2 like, Silent information regulator 2, SIR2, SIR2 like protein 2, Sirtuin type 2,SIRT2
Cellular localisation: Cytoplasm > cytoskeleton. Colocalizes with microtubules.

Molekulargewicht: 43kDa

Gen-ID: 22933, 466693

UniProt: Q8IXJ6