ESR2 Antikörper (C-Term)

Produktnummer ABIN7126353

Der Maus Monoklonal Anti-ESR2-Antikörper wurde für WB, IF, FACS und IHC (f) validiert. Er ist geeignet, ESR2 in Proben von Human zu detektieren.

Kurzübersicht für ESR2 Antikörper (C-Term) (ABIN7126353)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-ESR2 Antikörper

Bindungsspezifität: C-Term

Spezifität: This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide (CGMKMETLLPEATMEQ ) from the C terminus of the human estrogen receptor beta 2 isomer

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (2-4 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Human testis tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: ESR2

Hintergrund: Estrogen Receptor 2, Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2), Oestrogen Receptor Beta, Estrogen Receptor Beta, ER-BETA, ESTRB,ER-beta (Estrogen Receptor beta-2)
Cellular localisation: Nuclear

Molekulargewicht: 59kDa

Gen-ID: 2100, 660607

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling