Rekombinanter Estrogen Receptor alpha Antikörper (C-Term)

Produktnummer ABIN7126349

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch Estrogen Receptor alpha in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter Estrogen Receptor alpha Antikörper (C-Term) (ABIN7126349)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: ESR1-4039R

Produktdetails anti-Estrogen Receptor alpha Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Recombinant Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody

Spezifität: Recognizes a protein of 67 kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified by Protein A/G.

Immunogen: Recombinant fragment corresponding to C-terminus of human ER-α

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Humanbreast or endometrial carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: ESR1

Hintergrund:

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform,Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1

Humanbreast or endometrial carcinoma.

Molekulargewicht: ~67kDa

Gen-ID: 2099, 208124

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly