EIF4A2 Antikörper

Produktnummer ABIN7126324

Dieses Anti-EIF4A2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von EIF4A2 in ELISA und Coat. Geeignet für Human.

Kurzübersicht für EIF4A2 Antikörper (ABIN7126324)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF4A2 Antikörper ist unkonjugiert

Applikation: ELISA, Coating (Coat)

Produktdetails anti-EIF4A2 Antikörper

Spezifität: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5'-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5' cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Kreuzreaktivität (Details): Human. Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human EIF4A2 protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order Ab without BSA),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Andere Bezeichnung: EIF4A2

Hintergrund: ATP dependent RNA helicase eIF4A 2, BM-010, DDX2B, EIF4F, eukaryotic translation initiation factor 4A isoform 2, N-terminally processed,EIF4A2 (Eukaryotic Initiation Factor 4A-II)
Cellular localisation: Nucleus.

Molekulargewicht: 46kDa

Gen-ID: 1974

UniProt: Q14240