EIF2C3 Antikörper

Produktnummer ABIN7126312

Produktdetails anti-EIF2C3 Antikörper

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF2C3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS)

Spezifität: This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as argonaute RISC catalytic component 3.Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human AGO3 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF7 or U-2OS cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu EIF2C3

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Andere Bezeichnung: AGO3 (EIF2C3 Produkte)

Synonyme: EIF2C3 antikoerper, Eif2c3 antikoerper, Argonaute3 antikoerper, ago3 antikoerper, eif2c3 antikoerper, eif2c3b antikoerper, si:dkey-3n22.3 antikoerper, AW048688 antikoerper, C130014L07Rik antikoerper, ARGONAUTE 3 antikoerper, T19E23.8 antikoerper, T19E23_8 antikoerper, argonaute 3, RISC catalytic component antikoerper, argonaute RISC catalytic component 3b antikoerper, argonaute RISC catalytic subunit 3 antikoerper, argonaute RISC catalytic component 3 antikoerper, ARGONAUTE 3 antikoerper, AGO3 antikoerper, Ago3 antikoerper, ago3b antikoerper

Hintergrund: Argonaute 3, Argonaute3, eIF 2C 3, eIF-2C 3, eIF2C 3, Eif2c3, EIF2C3 protein, Eukaryotic translation initiation factor 2C 3, Eukaryotic translation initiation factor 2C3, FLJ12765, hAgo3, MGC86946, Protein argonaute-3,AGO3 (Argonaute-3) / eIF2C3
Cellular localisation: Cytoplasm>P-bodies. Nucleoplasm.

Molekulargewicht: 97.36kDa

Gen-ID: 192669, 657659

UniProt: Q9H9G7

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung