DCP2 Antikörper

Produktnummer ABIN7126292

Dieser Maus Monoklonal Antikörper detektiert spezifisch DCP2 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für DCP2 Antikörper (ABIN7126292)

Target: DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DCP2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: PCRP-DCP2-1D6

Produktdetails anti-DCP2 Antikörper

Verwendungszweck: DCP2 (Decapping mRNA 2) Antibody

Spezifität: The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3'exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay

Kreuzreaktivität (Details): Human. Predicted to react with Mouse and Rat.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human DCP2protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), ,Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: MCF7, Daudi or Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu DCP2

Target: DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))

Andere Bezeichnung: DCP2

Hintergrund:

DCP2 decapping enzyme homolog (S. cerevisiae), DFLJ33245, hDpc, m7GpppN-mRNA hydrolase, mRNA decapping enzyme 2, nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20)

Daudi or Jurkatcells., MCF7

Molekulargewicht: 48.4kDa

Gen-ID: 167227, 443875

UniProt: Q8IU60