DAZL Antikörper (C-Term)

Produktnummer ABIN7126282

Produktdetails anti-DAZL Antikörper

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DAZL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

Kreuzreaktivität (Details): Human, Mouse, Rat and Cynomolgus monkey.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human testis or ovary tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu DAZL

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Andere Bezeichnung: DAZL (DAZL Produkte)

Hintergrund: Deleted in azoospermia-like, DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, DAZH, DAZL1, DAZLA, SPGYLA,DAZL (Deleted in Azoospermia-like)
Cellular localisation: Cytoplasmic

Molekulargewicht: 33kDa

Gen-ID: 1618, 131179

UniProt: Q92904